Application

Cytopathic effect inhibition assay: 2 x 10E5 neutralization units per mL of antiserum. One neutralization unit is the amount of antiserum required to neutralize 1 unit of mouse IFN-beta. This material is prepared specifically for effective neutralization of mouse IFN-beta.

Western blot (1:500) The antibody reacts with a 19kDa band coresponding to Mu-IFN-beta. Protocol below:

1. Put protein (approx 50ng) in blocking solution for 30-60 min RT

2. Add Primary antibody (1:500) to blocking solution

3. Agitate membrane for 60 min, RT

4. Wash 3X for 5min each with PBS-Tween

5. Add secondary antibody (1:1000) after diluting in PBS-Tween

6. Gentle agitation for 60min.

7. Wash 3X for 5min each with PBS-Tween

8. Develop with reaction kit of choice

Immunoprecipitation

Immunohistochemistry

ELISA

Optimal working dilutions must be determined by end user.

Research Sub CategoryCytokines & Cytokine Receptors

Research CategoryInflammation & Immunology

Detect Interferon-β using this Anti-Interferon-β Antibody validated for use in ELISA, IP, WB, IH, NEUT.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Mouse IgG, whole molecule.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

2x10(e4) units

100 µL in 2x10(e4) units

Physical form

Rabbit polyclonal serum in buffer containing 0.3 M NaHCO3 and 200 mM NaCl.

Specificity

Reacts with mouse Interferon beta (IFNbeta). No reactivity is seen with IFNalpha or gamma

Storage and Stability

Maintain frozen at -20°C or below for retention of full activity, for up to 12 months. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum.